Product Description |
Recombinant CHO-K1 cells
stably overexpress G15 alpha subunit protein (Gα15), which belongs to
Gq/11/14/15 alpha subunit family. When Gα15 and Gs-coupled or Gi/o-coupled
receptor are co-expressed in cells, the cells not only can be triggered to
stimulate or inhibit the cAMP pathway based on the coupled G alpha subunit
types, but also can conduct Gq-coupled signal transduction and mobilize
intracellular calcium following certain ligand binding. This cell line is
recommended to use as a host cell line for transient or stable expression of Gs
and Gi/o -coupled receptors, which is able to provide more readouts to study G
protein-coupled receptors. |
Expressed Gene |
Codon Optimized from NM_002068; no expressed tags |
Target Protein |
NP_002059 |
Host Cell |
CHO-K1 |
Quantity |
2 vials of frozen cells (>1X106 per
vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative. The mycoplasma test was performed with
MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile. |
Culture Properties |
Adherent |
Freeze Medium |
45% complete growth medium, 45% FBS (Cat. No. 10099-141,
Gibco), 10% DMSO (Cat. No. D2650, Sigma) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium |
Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco), 100 μg/ml Hygromycin B (Cat. No. 10687010,
Invitrogen) |
Gene Synonyms |
GNA15, GNA16 |
Background |
The sequence of Gα15 ATGGCCCGCTCGCTGACCTGGCGCTGCTGCCCCTGGTGCCTGACGGAGGATGAGAAGGCCGCCGCCCGGGTGGACCAGGAGATCAA CAGGATCCTCTTGGAGCAGAAGAAGCAGGACCGCGGGGAGCTGAAGCTGCTGCTTTTGGGCCCAGGCGAGAGCGGGAAGAGCACC TTCATCAAGCAGATGCGGATCATCCACGGCGCCGGCTACTCGGAGGAGGAGCGCAAGGGCTTCCGGCCCCTGGTCTACCAGAACATC TTCGTGTCCATGCGGGCCATGATCGAGGCCATGGAGCGGCTGCAGATTCCATTCAGCAGGCCCGAGAGCAAGCACCACGCTAGCCTG GTCATGAGCCAGGACCCCTATAAAGTGACCACGTTTGAGAAGCGCTACGCTGCGGCCATGCAGTGGCTGTGGAGGGATGCCGGCAT CCGGGCCTACTATGAGCGTCGGCGGGAATTCCACCTGCTCGATTCAGCCGTGTACTACCTGTCCCACCTGGAGCGCATCACCGAGGAG GGCTACGTCCCCACAGCTCAGGACGTGCTCCGCAGCCGCATGCCCACCACTGGCATCAACGAGTACTGCTTCTCCGTGCAGAAAACCA ACCTGCGGATCGTGGACGTCGGGGGCCAGAAGTCAGAGCGTAAGAAATGGATCCATTGTTTCGAGAACGTGATCGCCCTCATCTACCT GGCCTCACTGAGTGAATACGACCAGTGCCTGGAGGAGAACAACCAGGAGAACCGCATGAAGGAGAGCCTCGCATTGTTTGGGACTAT CCTGGAACTACCCTGGTTCAAAAGCACATCCGTCATCCTCTTTCTCAACAAAACCGACATCCTGGAGGAGAAAATCCCCACCTCCCACC TGGCTACCTATTTCCCCAGTTTCCAGGGCCCTAAGCAGGATGCTGAGGCAGCCAAGAGGTTCATCCTGGACATGTACACGAGGATGTAC ACCGGGTGCGTGGACGGCCCCGAGGGCAGCAAGAAGGGCGCACGATCCCGACGCCTCTTCAGCCACTACACATGTGCCACAGACACA CAGAACATCCGCAAGGTCTTCAAGGACGTGCGGGACTCGGTGCTCGCCCGCTACCTGGACGAGATCAACCTGCTGTGA |
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