Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PAF. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PAF (Mean ± SD, n = 2). The EC50 of PAF on the cells was 0.13 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/PTAFR Stable Cell Line
The platelet-activating factor (PAF) receptor (PTAFR) is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and releases of cytokine and leukotriene (Stafforini et al., 2003). In humans, various diseases have been associated with PAF, such as allergic asthma, endotoxic shock, atherosclerosis and psoriasis.
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