Product Description |
Recombinant CHO-K1 cells
stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the
cell surface. The surface expression of VISTA is validated by FACS analysis.
This cell line is designed for cell-based binding for screening antibodies binding
with VISTA and evalsuating target binding affinity. |
Applications |
Cell-based binding assay or
use as immunogen |
Expressed Gene |
Codon
optimized from NM_022153.1; no expressed tags |
Target Protein |
NP_071436 |
Host Cell |
CHO-K1 |
Quantity |
2
vials of frozen cells (>1×106
per vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No.LT07-318, Lonza). |
Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile. |
Culture Properties |
Adherent |
Freeze Medium |
95% Complete growth medium,
5% DMSO (Cat. No.D2650, Sigma) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s) Medium
(Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No.10099-141,
Gibco) |
Culture Medium |
Ham’s F-12K (Kaighn’s)
Medium (Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No.10099-141,
Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Gene Synonyms |
B7H5, GI24, B7-H5, Dies1, PD-1H, SISP1, VISTA, PP2135,
C10orf54, DD1alpha |
Background |
V-domain
Ig suppressor of T cell activation (VISTA) is a potent negative regulator of
T-cell function that is expressed on hematopoietic cells. VISTA levels are
heightened within the tumor microenvironment, in which its blockade can enhance
antitumor immune responses in mice. In humans, blockade of the related
programmed cell death 1 (PD-1) pathway has shown great potential in clinical
immunotherapy trials. Here, we report the structure of human VISTA and examine
its function in lymphocyte negative regulation in cancer. |
Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).
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