Product Description |
Recombinant CHO-K1 cells
stably overexpress Homo sapiens programmed
death-ligand 1 (PD-L1) on the cell surface. The surface expression is validated
by FACS analysis. This cell line is recommended for cell-based binding assay to
screen antibodies against PD-L1 or to measure binding
affinity between PD-L1 and anti-PD-L1 antibodies. |
Applications |
Cell-based binding assay or use as immunogen. |
Expressed Gene |
Codon Optimized from NM_014143.3; no expressed tags |
Target Protein |
NP_054862.1 |
Host Cell |
CHO-K1 |
Quantity |
2 vials of frozen cells (> 1×106 per vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon receipt.
Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative. The mycoplasma test was performed with
MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza). |
Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile. |
Culture Properties |
Adherent |
Freeze Medium |
95% Culture Medium, 5% (V/V) DMSO (Cat. No.D2650,
Sigma) |
Complete Growth Medium |
Ham's F-12K (Kaighn's) Meidum (Cat. No. 21127-022, Gibco),
10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium |
Ham’s F-12K (Kaighn’s) Meidum (Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03,
Gibco) |
Gene Synonyms |
CD274;
B7-H; B7H1; PDCD1L1; PDCD1LG1; PDL1 |
Background |
Programmed death-ligand 1
(PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1
(B7-H1) is a protein that in humans is encoded by the CD274 gene. The formation
of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an
inhibitory signal which reduces the proliferation of these CD8+ T
cells at the lymph nodes and supplementary to that PD-1 is also able to control
the accumulation of foreign antigen specific T cells in the lymph nodes through
apoptosis which is further mediated by a lower regulation of the gene Bcl-2. |
Figure 1: FACS analysis of cell surface expression of PD-L1 on CHO-K1 cells. The CHO-K1/PD-L1 cells (red) and the negative control CHO-K1 cells (green) were probed using Anti-Human PD-L1 (GenScript) and Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).
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