Product Description |
Recombinant
CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated
protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line
is designed for cell-based binding for screening antibodies binding with mouse CTLA4
and for evalsuating target binding affinity. |
Applications |
Cell-based binding assay or use as immunogen |
Expressed Gene |
Codon
optimized from NM_009843.4; no expressed tags |
Target Protein |
NP_033973 |
Host Cell |
CHO-K1 |
Quantity |
2 vials of frozen cells (1x106 per vial in 1 mL) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative. The mycoplasma test was performed with
MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza). |
Stability |
Stable through more than 15 passages with no significant changes in
assay performance or expression profile. |
Culture Properties |
Adherent |
Freeze Medium |
95% Culture Medium, 5% (V/V) DMSO (Cat. No. D2650,
Sigma) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco),
10% FBS (Cat.
No. 10099-141, Gibco) |
Culture Medium |
Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin
(Cat.
No. A11138-03, Gibco) |
Gene Synonyms |
Cd152; Ly-56; Ctla-4 |
Background |
CTLA4
or CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152
(cluster of differentiation 152), is a protein receptor that, functioning as an
immune checkpoint, downregulates immune responses. CTLA4 is constitutively
expressed in Tregs but only upregulated in conventional T cells after
activation. It acts as an "off" switch when bound to CD80 or CD86 on
the surface of antigen-presenting cells. |
Figure 1: FACS analysis of cell surface expression of mouse CTLA4 on CHO-K1/mouse CTLA4 cells. The CHO-K1/mouse CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE anti-mouse CD152 (Cat. No. 106305, Biolegend).
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