Product Description |
Recombinant CHO-K1 cells
stably overexpress human immunoglobulin gamma Fc region receptor II-a
(FcγRIIa / CD32A 131His). The surface
expression of CD32A 131His is validated by FACS analysis. This stable cell line
product is designed for measuring binding affinity and stability of Fc region
of antibodies and Fc fusion proteins, or antibody based biologics binding with
CD32A 131His. GenScript also offers CD32A
131Arg stable cell line (Cat. No. M00887) for FcγRIIa
polymorphism study. |
Applications |
Cell-based binding assay; use as immunogen for antibody
development |
Expressed Gene |
Codon optimized from NM_001136219.1; no expressed tags |
Target Protein |
NP_001129691.1 |
Host Cell |
CHO-K1 |
Quantity |
Two
vials of frozen cells (>1×106 per vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
Culture Properties |
Adherent |
Freeze Medium |
95% complete growth medium,
5% DMSO (Cat. No. D2650, Sigma) |
Complete Growth Medium |
Ham’s F-12K (Kaighn’s)
(Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium |
Ham’s F-12K (Kaighn’s), 10%
FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Gene Synonyms |
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1 |
Background |
CD32
is a surface receptor protein and part of a large population of B cell
co-receptors, which act to modulate signaling. It has a low-affinity for IgG
antibodies and down-regulates antibody production in the presence of IgG. This
feedback loop acts to lower the production of IgG by B cells when there is a
surplus in the body. Monoclonal antibodies can distinguish between CD32A and
CD32B. |
Figure 1: FACS analysis of cell surface expression of human CD32A 131His on CHO-K1 cells.
CHO-K1/human CD32A 131His cells (blue) and negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Biosescience).
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