Product Description |
Recombinant CHO-K1 cells
stably overexpress Homo sapiens
cell-type natural killer cells Fc gamma receptor IIc1 (FcγRIIc
/ CD32C) on the cell surface. The surface expression of FcγRIIc
is validated by FACS analysis. This stable cell line is recommended for
evalsuating the binding affinity between FcγRIIc
and Fc region of antibodies, Fc fusion proteins, or antibody-based biologics. |
Applications |
Cell-based binding assay; use as immunogen for
antibody development |
Expressed Gene |
Codon
optimized from U90938.1; no expressed tags |
Target Protein |
AAC12807.1 |
Host Cell |
CHO-K1 |
Size |
Two vials of frozen cells (>1×106 per
vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile. |
Culture Properties |
Adherent |
Freeze Medium |
95% Complete Growth Medium, 5% DMSO (Cat. No. D2650,
Sigma) |
Complete Growth Medium |
Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
Culture Medium |
Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022,
Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03,
Gibco) |
Gene Synonyms |
CD32;
FCG2; CD32C; CDW32; IGFR2; FCRIIC |
Background |
Human studies suggest
expression of CD32C could help predict patient responses to vaccines. CD32C
normally contains a termination codon, but 7%–15% of individuals carry a
mutation that changes it into a full-length open reading frame. Individuals
with this variant expressed CD32C on B cells and were more likely to respond to
an anthrax vaccine than patients that did not express the variant. Individuals
with the full-length CD32C variant also were more likely to develop the
autoimmune disease systemic lupus erythematosus (SLE) than those without the
variant. |
Figure 1: FACS analysis of cell surface expression of CD32C 13Gln on CHO-K1 cells. The CHO-K1/CD32C 13Gln cells (blue) and the negative control CHO-K1 cells (red) were stained with APC Mouse Anti-Human CD32 (Cat. No. 559769, BD Biosesciences).
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