Product Description |
Recombinant
CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb
/ CD16B NA1). The surface expression of CD16B NA1 is validated by FACS
analysis. This stable cell line product is designed for measuring binding
affinity and stability of Fc region of antibodies, Fc fusion proteins, or
antibody based biologics that bind with CD16B during biologics research and
development. |
Applications |
Cell-based binding assay; use as immunogen for antibody
development |
Expressed Gene |
Codon
optimized from CCDS41433.1; no expressed tags |
Target Protein |
NP_000561.3 |
Host Cell |
CHO-K1 |
Quantity |
2 vials of frozen cells (>1×106
per vial in 1 ml) |
Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
Mycoplasma Status |
Negative.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
Culture Properties |
Adherent |
Freeze Medium |
95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
Complete Growth Medium |
Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10%
FBS (Cat. No.10099-141, Gibco) |
Culture Medium |
Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10%
FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco) |
Gene Synonyms |
CD16;
FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb |
Background |
CD16 has been
identified as FcγRIIIa (CD16A) and FcγRIIIb (CD16B) which are similar to each
other. CD16A exists as a heterooligomeric polypeptide-anchored form in
macrophages and NK cells. CD16B exists as a monomeric
glycosylphosphatidylinositol (GPI)-anchored form in neutrophils [1]. CD16B (FcγRIIIb)
exists in two allelic forms, NA1 and NA2, [2-3]. The polymorphism of CD16B
exhibits different binding affinities for IgG1 and IgG3 subclasses [3]. The
different allelic versions are also demonstrated to be associated with
susceptibility to diseases like idiopathic pulmonary fibrosis [4] and systemic
lupus [5]. |
Figure 1: FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells.
The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (blue) were stained with PE anti-human CD16 antibody (Cat. No. 302008, BioLegend).
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