Description
4-1BB is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), CD137 and induced by lymphocyte activation (ILA). It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule.
4-1BB can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, 4-1BB expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
The best characterized activity of 4-1BB is its costimulatory activity for activated T cells. Crosslinking of 4-1BB enhances T cell proliferation, IL-2 secretion, survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
HT1080 is derived from human fibrosacoma biopsy. It is highly active in NF-kB signaling pathway which is necessary for immune responses. HT1080 cells overexpressed with 4-1BB are significantly increased in immune response indicated by elevated production of IL-8.
|
Applications |
Reporter cell line for 4-1BB functional assay. Assay readout IL-8. |
Storage |
Liquid nitrogen immediately upon receiving |
QC |
Mycoplasma negative, stability tested for 20 passages |
Species |
Human |
Stability |
tested for 20 passages |
Volume |
1ml in each vial, 2 vials |
Freeze Medium |
95% complete growth medium, 5% DMSO |
Culture Medium |
MEM, 10% FBS, 200 μg/ml Hygromycin B, 2 μg/ml Puromycin |
Flow cytometry analysis of 4-1BB protein expression in HT1080/4-1BB cells.
Expression of 4-1BB was detected with anti-4-1BB antibody.
Red: HT1080 cells,
Blue: HT1080/4-1BB cells.
Functional analysis of 4-1BBL on HT1080/4-1BB cells by measuring IL-8 release.
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