Description
Claudins are a family of proteins, which play a role in maintaining tight cell junctions. Differentclaudin subtypes are expressed on different tissues.Claudin18 isoform 2 (CLDN18.2) expressionin normal tissues is restricted to cells of the gastric mucosa and is absent from other healthytissues However, CLDN 18.2 is expressed in 70% of primary gastric adenocarcinomas and itsmetastases (101). It has also beenshown to be expressed in a number of other cancers,including pancreatic (50%), esophagus (30%) and NSCLC (25%)The restricted expressionmakes it a potential drug target for the treatment of gastric and pancreatic adenocarcinoma, asevidenced by effortsto target CLDN18.2 via naked antibody and CAR-T modalities. * The mycoplasma test was
performed with MycoAlert™ PLUS Mycoplasma Detection Kit of Lonza. |
Note
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Applications |
Binding assay or use as
immunogen |
Storage |
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. We recommend immediate resuscitation upon receipt of the cells |
QC |
Mycoplasma negative |
Stability |
Stable expression of
CLDN18.2 for more than 15 passages |
Freeze Medium |
49% DMEM, 45% FBS, 6% (V/V)
DMSO |
Culture Medium |
DMEM, 10% FBS,500μg/ml(G418 Sulfate) Geneticin (G418 Sulfate) |
FACS analysis of CLDN18.2 expression in HEK293/ CLDN18.2 clone by antiCLDN18.2, compared with blank control (green line, untreated cell) and negative control (blue line, only treated with goat anti-human antibody). The cells analyzed are live cells (E1 gate).
Stability testing of CLDN18.2 expression in HEK293/ CLDN18.2 clone.for 20 passages by anti-CLDN18.2, compared with blank control (blue line, untreated cell) and negative control (purple line, treated with goat anti-human antibody)
如您需要获取细胞系产品的说明书,请点击“Manual”,完善信息后点击“提交”。
我们会第一时间联系回复您。注意:中国地区购买的产品,说明书需要以中文站为准。
您也可拨打400-0258686转5810,或发邮件至product@lishanghui.com163am银河线路娱乐官网。
更多产品文件类型 >>